AUTOPHAGOSOME CONTENT PROFILING USING PROXIMITY BIOTINYLATION PROTEOMICS COUPLED TO PROTEASE DIGESTION IN MAMMALIAN CELLS

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells

Summary: The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein 100w products of interest by proximity-dependent biotinylation.Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes and potentially other short

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A case report of acute dermatitis that developed during an experiment examining the bromination of 3-hexylthiophene

Abstract Occupational kenya tree coral for sale cases with allergic reaction to fragrance substances, which refer to various chemicals providing aroma characteristics, are arising with its recent usage diversification from pharmaceutical, perfume industry to aromatic remedies.However, chemicals responsible for fragrance allergy have hardly been ide

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